The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Fluorescence Microplate Reader Comparison Chart. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Selects either 96-well or 384-well plate for a stand-alone read. 057, depending on lot number. x. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Multimode Microplate Reader. Supplier: Molecular Devices. (40 µl maximum) Clear, white, or black. Find our technical support information along with contact details. x. Gemini EM Reader scans. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Streamline your workflow with comparative confluence assays to identify and verify gene edits. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Reading Capabilities: cuvette or microplate. ZERO BIAS - scores, article reviews, protocol conditions and more. Danaher Inc 384 well plate 384 Well Plate, supplied by Danaher Inc, used in various techniques. Optional. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels. #0200-7011) Instrument setupRound well design with a solid black flat bottom2. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. 20 attomol ATP per well. QC tools and IQOQ services. Service Contracts, Preventative Maintenance and. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Select Update Driver. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 5–25 µl. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. External Dimensions without lid (mm). A. FlexIII. In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. Microplates for applications that require high sample recovery rates. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. After the reaction was complete, 5 µL of each sample was transferred into wells of a 384-well plate and detected on the microplate readers using the instrument settings listed in Table 1. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. All stand-alone instrument functions can be controlled using the software. smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. x. The SpectraTest validation. 2. 6-384. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. Get Quote . 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. 003 fmol 0. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. Minimum volume and maximum throughput with 96- or. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. +1 800. Tb. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. flat meniscus), but is approx 15 mL. X. both 96- and 384-well plates and comes with four wash/rinse bottles and one waste. 12, 24, 96 & 384-well SBS microplates; Range of 96, 384-well SBS microplates . Biotek Synergy 2 Plate Reader Multi-Mode. Determine concentration using the Beer-Lambert LawMolecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. #S1205. 5 ng per well. If you have an xml plate object, then. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. #3-000-510)Page 154: Ordering Parts And Accessories. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. These models can no longer be repaired. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. 020 fmol 0. Time-resolved phosphorescence. )Step 4: Incubate at room temperature for 1 hour, protected from light. Published Dec 16, 2020 | Updated Dec 16, 2020. The small-footprint Multiwash+ Microplate Washer quickly washes with both 96 and 384-well plates, has 20 standard wash protocols, and a simple LCD screen interface to customize speed, volume, soak time, and aspiration settings. VIS absorbance detection. for ELISAs and most cell-based assays. x. Alpha 1536 HTS 96, 384, and . For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. The opaque walls prevent stray light from being detected from neighbouring. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. ability to use as little as 2˜µL samples with 24- or 64-well plates. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. Home > Search Results > Molecular Devices LLC > 384 well plate. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 2. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 190. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. 100 fmol luciferase per well. Can read 96 well plates (optional 384 well). $245. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Dynamic range > 6 decades . After reading the ELISA plate on both readers, each standard curve was nearly identical. Description. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. This reader also makes use of a unique temperature. . After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Validation made easy -The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. These lab workhorses are used in drug discovery, research, bioassay validation, and. Molecular Devices: n/a:. UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. USA. Experience the identifiably different. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. #3635) Transferpette micropipette (Drummond Scientific cat. $402. Features. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. Next-generation luminescence plate reader. The plate reader optics are tunable for. x. 1 a. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. 384- or 96-well read plate containing cells loaded. We would like to show you a description here but the site won’t allow us. Molecular Devices Spectramax. A 96- and 384-well automated washer. M5. 0200-6262. 1. Shaking: Linear, orbital, and double orbital. Guaranteed sensitivity: < 100 amol of biotinylated-LCK-P peptide, 25µL/well in a 384-well plate. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Add 10 µL of ADP Detection Mix to those rows. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Pick the best speed, sensitivity, and price for your needs. There is no product in the shopping cart, buy it!. In simplest terms, a plate reader measures and. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. A thermal cycling reaction was immediately initiated. 039. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. UV absorbance detection. Experience the identifiably different. For SpectraMax L or LMax II, LMax II 384 - no adapter used. EMax. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. x. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. 100 fmol 384-well (75 µL) 0. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Outer wells aligned for proper viewing with FLIPR. 96- and 384-well microplates. Instrument pricing. 384-well TC plate, Poly-d-Lysine, Flat. Compare specifications and request a quote from leading suppliers. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. x. 306 plate air cal. Technical specifications. Supplier: Molecular Devices. Get Quote . #ID5) HTRF Detection System (Molecular Devices cat. within each well. #i3X) with: SoftMax Pro 7. Also refer to this Multimode Reader Comparison. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells. Each kit is sufficient. Microplates, cuvettes (via adapter) Detectors. Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. 314 low lamp level warning. FlexIII. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Test up to 3. Figure 2-23: Bottle holder To install the bottle holder: 1. 8 mm distance between well bottoms to plate bottom. Contact our support team with your questions regarding Molecular Devices products and applications. M2. The former have been the traditional domain of plate readers and great strides have been. Analyst, M5, Flex 3, and SpectraMax L readers work differently. High base plate design is compatible with automated handling (gripping) and bar coding. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. 2. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . DynaPro ™ Plate Reader III. 304 signal level saturation. Our microplate. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. For Raman excitation, a continuous-wave (CW) laser at 532 nm (Verdi V-18, Coherent) was used. 40266. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 96- and 384-well microplates. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. ". Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. 58% for the detection module and 2. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. Plate Formats: 6- to 384-well plates. 130009WThe SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. VMax . SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. T = I/I 0 and %T = 100 (T). Products : Ionworks HT, Ionworks Quattro. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modefor the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 384-well format. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6 to 384 wells, the SpectraMax iD3 reader is the complete solution. Bioz Stars score: 86/100, based on 1 PubMed. com Check our website for a current listing of worldwide distributors. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andThe Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. SpectraDrop High-Throughput Screening (HTS) Kit. Algorithms are optimized for accurate cell detection and. Category 2 and 3 microplate readers use gradient heating as well. Following the flushing of the machine as described in Section 3. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. 20 µL (96-well plates) or 10 µL (384-well plates. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Microplate Readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 3. Use standard plate unless you see your type listed. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Using Molecular Devices microplate readers and SoftMax. It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. MultiWash+ Microplate Washer. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Turn the. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. Scan every well . SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The plate reader can measure multiple readouts, including UV and. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. 2. The modular platform allows upgrading to expand functionality as your laboratory workflows change. ©2010 Molecular Devices, Inc. San Jose, CA 95134. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. 4 Equipment. Streamline GLP/GMP compliance. The system provides automation for up to 50 microplates for easy walkaway automation. #PLUS 384) UV-transparent 96-well microplates (Corning cat. 5 mP 1536–2. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Note: These settings are based on necessary cookies. It features a modular design that allows users to add or change detection cartridges according to their needs. Step 1: Open the SoftMax Pro IMAP FP protocol. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. We would like to show you a description here but the site won’t allow us. 6-384 (96- and 384-well with pipetting) 96-well microplates. 20 attomol ATP per well. 96-, 384-, and 1536. 76 ± 0. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. When the user applies a set temperature of 37°C, the top is heated to 37. Time-resolved phosphorescence. Plus 384. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). 4 Equipment. Source plate capacity; 1 x plates. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. If the computer is connected to the Internet: Select Search Automatically for. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Molecular Devices has provided scientists with tools to expand the boundaries of their research. EMax. The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. Artel MVS Bundle DM Std/800TSNB Reader Details. X. Versa Max. x. ,LTD (CAT#: STEM-S. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. A control well was set up to verify. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. –Min. Not for use in diagnostic procedures. 96- and 384-well . B. run on the FlexStation 3 reader in 384-well microplate with the FLIPR® Calcium 4 Assay Kit. x. VIS absorbance detection. Select Yes at the warning. All stand-alone instrument functions can be controlled using the software. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. By measuring optical pathlength, it. NanoQuant plate. Select Start>Control panel>Device Manager. An industry-renowned instrument for monitoring GPCRs and ion channels. $913. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Two high-energy xenon flash lamps: Reading capabilities. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. That explains the lower values on the LmaxII384 plate reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 19 H mm that meet microplate industry standards. x. 2. Industry standard disposable (choose from recommended list). The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. EMax. x. * Note: Programming the washer with a final aspirate height of 4. Two high-energy xenon flash lamps: Reading capabilities. • Easy and gentle immobilization of biotin. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Pre-read measurements were made with 200 µL water in each well of a Costar UV plate, after which the plate was emptied and blotted dry. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. #6590-0118) Filter 665/10 (Molecular Devices cat. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. 5in (46. Read the microplate. 7. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. B. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (T onset, T m, T agg) and colloidal stability (A 2 /B 22,. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. In the M2, M5 and Plus. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. The fluorescent intensity of each well was measured by using a fluorescence plate reader (SpectraMax Paradigm, Molecular Devices, Sunnyvale, CA, USA) at 588-nm excitation and 633-nm emission. Molecular Devices products are warranted to meet the stated specifications. x. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Related products. 384-Well Microarray/High Sample Recovery Plate.